Biosensor Nanomaterials by Songjun Li, Jagdish Singh, He Li, Ipsita A. Banerjee

By Songjun Li, Jagdish Singh, He Li, Ipsita A. Banerjee

Concentrating on the fabrics appropriate for biosensor functions, resembling nanoparticles, quantum dots, meso- and nanoporous fabrics and nanotubes, this article permits the reader to arrange the respective nanomaterials to be used in genuine units by means of applicable functionalization, floor processing or directed self-assembly. the most detection tools used are electrochemical, optical, and mechanical, offering strategies to hard tasks.The result's a reference for researchers and builders, disseminating first-hand info on which nanomaterial is most suitable to a selected software -- and why.

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A way to obtain highly ordered vertical PANI nanotubes was developed by Wang et al. [112] using anodic aluminum oxide membrane as template and electropolymerizing the aniline monomers inside the pores by cyclic voltammetry. An amperometric glucose biosensor was then realized by electrostatically incorporating GOx (negatively charged at physiologic pH) in positively charged PANI nanotube inner walls. 5 mM) was achieved. Moreover the biosensor resulted selective towards common interfering species (ascorbic acid, uric acid, 4-acetamidophenol).

The sensors were realized by thermal CVD onto insulators (SiO2, Si3N4) as well as metallic substrates using acetylene and ammonia as precursor gases, and the best performance was obtained with nanotubes grown on an aluminum layer able to serve as growing substrate as well as electrical contact. Combining such an electrode platform with covalent DNA immobilization on the open ends of the tubes and enzyme labeling of the hybrid, a detection limit in the nanomolar range of oligonucleotide target was achieved.

1 ng ml−1 using oligonucleotides functionalized with polymeric beads carrying Au-NPs. This strategy involved the hybridization of the oligonucleotide probe (captured on magnetic beads) to the DNA target labeled with the gold-loaded sphere, and subsequent dissolution and stripping-potentiometric detection of the gold tracer. Lermo et al. [173] developed a genomagnetic assay for the detection of food pathogens based on a graphite/epoxy composite magneto electrode as electrochemical transducer. The assay was performed in a sandwich format by double labeling the amplicon ends during PCR, with a biotinylated capture probe, to achieve the immobilization on streptavidin-coated magnetic beads and with a digoxigenin signaling probe, to achieve further labeling with the enzyme marker (antidigoxigenin HRP).

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